Relationships are calculated on the basis of ANI (average nucleotide identity) and AAI (average amino acid identity). MiGA makes inferences based on the obtained values and predetermined thresholds for each taxonomic rank. For instance, if a given sequence shares at least 95% ANI with a reference genome, there is a high likelihood that it should be assigned to the same species. For more divergent query sequences AAI, is used. Genome sequences are hypothesized to be from the same genus if they have an AAI above 65%. If the sequences show AAI between 45 and 65%, they are hypothesized to be from the same family. Further information on this approach and thresholds used can be found by clicking on the information icons (blue circles with i's in them) next to each title.